منابع مشابه
Gene targeting in immunology.
Transgenics and gene targeting by homologous recombination provide an ideal opportunity to delineate immune functions of specific genes. These gene knockout mice are powerful tools to understand complex mechanism of immune system and molecular processes underlying autoimmune diseases and immunodeficiencies. Availability of an increased pool of genetically manipulated mice will provide a basic f...
متن کاملKidney-specific gene targeting.
Gene targeting in mice is a powerful tool for identifying the in vivo functions of proteins and for producing new animal models of human diseases. In the conventional approach, the gene encoding a protein of interest is disrupted by homologous recombination in embryonic stem (ES) cells. Targeted ES cells are injected into blastocysts to produce chimeric mice, which are then bred to produce knoc...
متن کاملGene Targeting in Hemostasis
1. Abstract 2. Introduction 3. Hepsin cDNA and gene structures 3.1. Cloning of hepsin cDNA 3.2. Hepsin gene structure 3.3. The promoter sequence of the mouse hepsin gene. 4. Hepsin protein 4.1. The domain structure of hepsin 4.2. Biochemical properties of hepsin 4.2.1. Molecular weight 4.2.2. Membrane topology 4.2.3. Substrate specificity 4.2.4. Effect of inhibitors 5. Functions of hepsin 5.1. ...
متن کاملGene targeting in Arabidopsis.
Precise modification by gene targeting (GT) provides an important tool for studies of gene function in vivo. Although routine with many organisms, only isolated examples of GT events have been reported for flowering plants. These were at low frequencies precluding reliable estimation of targeting efficiency and evaluation of GT mechanisms. Here we present an unambiguous and straightforward syst...
متن کاملIn planta gene targeting.
The development of designed site-specific endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different species. However, the methods described in plants require a highly efficient transformation and regeneration procedure and, therefore, can be applied to very few species. Here, we describe a highly efficient GT system that is suitable for all transformable pl...
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ژورنال
عنوان ژورنال: Cell
سال: 2004
ISSN: 0092-8674
DOI: 10.1016/j.cell.2004.07.018